Decoding Fiber photometry & Rat behavior
Title
Decoding the bulk signal from fiber photometry during rat behavior
Leaders
Maya Williams, Mickael Degoult, Christelle Baunez
Collaborators
Nicolas Maurice, Lucie Vignal
Brainhack Global 2022 Event
Brainhack Marseille
Project Description
What are you doing, for whom, and why? I am collecting calcium signals from Infalimbic, Prelimbic, and Anterior cingulate projection terminals at the subthalamic nucleus (STN) using fiber photometery. The goal is to look at the signal from these regions during sucrose and cocaine self administration, to discover the role of these pathways in food vs cocaine intake. In addition, we would focus on discrete behavior during the trails ( lever pressing, reward delivery, error responses) to study the signals of these pathways during both sucrose and cocaine intake.
What makes your project special and exciting? It will be the first time the hyperdirect pathway is studied in such detail, looking at the mulitpule cortical projection the STN and comparing their roles in food vs cocaine rewards. From perovious work in the lab, we know that STN lesions have opposite effects on cocaine and food intake, increasing food intake while decreasing cocaine intake. This study will explore the much less studies hyperdirect pathway and its role in addiction like behaviors so that the STN can be consided a target for theraputic treatment in addiction, without harming a persons natural motivations for food.
How to get started? I have collected data from sucrose taking rats, and addapted an open souce code to fit my data. I next need help on further analysis, combinding results into groups, and doing statistical analysis.
Where to find key resources? some codes I have used or wish to try: https://www.nature.com/articles/s41598-021-03626-9 https://pubmed.ncbi.nlm.nih.gov/31680685/
Link to project repository/sources
https://github.com/MayaIwilliams/Fiber_processing % my data and modified code https://github.com/LernerLab/GuPPy % GUI developed by a different lab. I have not tested yet
Goals for Brainhack Global
increase matlab literacy OR switch to python save output and compare traces
Good first issues
issue one: How is my code? can i make it more simple
issue two: How is the GUI working, can I use it for my data?
Communication channels
NA
Skills
80% matlab coding 20% teaching for dummies
Onboarding documentation
No response
What will participants learn?
Fiber photometry techniques and signal processing
Data to use
No response
Number of collaborators
1
Credit to collaborators
project contibuters will be listed in Readme
Image
Leave this text if you don’t have an image yet.
Type
coding_methods, method_development
Development status
1_basic structure
Topic
neural_decoding, other
Tools
other
Programming language
Matlab, Python
Modalities
other
Git skills
0_no_git_skills
Anything else?
No response
Things to do after the project is submitted and ready to review.
- Add a comment below the main post of your issue saying:
Hi @brainhackorg/project-monitors my project is ready! - Twitter-sized summary of your project pitch.